FB-6000-45
Fisher BioReagents™TaqDNA Polymerase
Manufacturer: Fischer Scientific
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Composition
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl225mM solution in a separate vial
Description
Taq DNA Polymerase
Format
Poly Tube
For Use With (Application)
Standard PCR
Storage Buffer
Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Content And Storage
-20°C
Form
Liquid
Quantity
100 Units
Concentration
5U/μL
Related Products
Description
- Licensed for use in PCR Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E
- coli host Has a 5«→3« exonuclease activity Available with two different reaction buffers Buffer A contains an optimized final concentration of 15mM MgCl 2 for most PCR amplifications Buffer B does not contain MgCl 2 ; MgCl 2 is provided in a separate vial as a 25mM solution Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™ Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl 2 ) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl 2 solution in a separate vial Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above
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Composition: 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl225mM solution in a separate vial
Description: Taq DNA Polymerase
Format: Poly Tube
For Use With (Application): Standard PCR
Storage Buffer: Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Content And Storage: -20°C
Form: Liquid
Quantity: 100 Units
Concentration: 5U/μL
Composition:
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl225mM solution in a separate vial
Description:
Taq DNA Polymerase
Format:
Poly Tube
For Use With (Application):
Standard PCR
Storage Buffer:
Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Content And Storage:
-20°C
Form:
Liquid
Quantity:
100 Units
Concentration:
5U/μL
Composition: 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl225mM solution in a separate vial
Description: Taq DNA Polymerase
Format: Poly Tube
For Use With (Application): Standard PCR
Storage Buffer: Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Content And Storage: -20°C
Form: Liquid
Quantity: 5 × 100 Units
Concentration: 5U/μL
Composition:
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl225mM solution in a separate vial
Description:
Taq DNA Polymerase
Format:
Poly Tube
For Use With (Application):
Standard PCR
Storage Buffer:
Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Content And Storage:
-20°C
Form:
Liquid
Quantity:
5 × 100 Units
Concentration:
5U/μL
Composition: 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl225mM solution in a separate vial
Description: Taq DNA Polymerase
Format: Poly Tube
For Use With (Application): Standard PCR
Storage Buffer: Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Content And Storage: -20°C
Form: Liquid
Quantity: 5 × 500 Units
Concentration: 5U/μL
Composition:
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl225mM solution in a separate vial
Description:
Taq DNA Polymerase
Format:
Poly Tube
For Use With (Application):
Standard PCR
Storage Buffer:
Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Content And Storage:
-20°C
Form:
Liquid
Quantity:
5 × 500 Units
Concentration:
5U/μL
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