Fisher BioReagents™TaqDNA Polymerase

Description

• Licensed for use in PCR Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E
• coli host Has a 5«→3« exonuclease activity Available with two different reaction buffers Buffer A contains an optimized final concentration of 15mM MgCl 2 for most PCR amplifications Buffer B does not contain MgCl 2 ; MgCl 2 is provided in a separate vial as a 25mM solution Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™ Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl 2 ) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl 2 solution in a separate vial Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above