Thermo Scientific™ dsDNase

Description

• Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples
• It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphate and 3'-hydroxyl termini
• Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA such as cDNA and primers are not cleaved
• dsDNase is easily inactivated by modetate heat treatment (55°C)
• These features make dsDNase an excellent choice for gDNA elimation prior reverse transcription
• It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure
• Highlights Highly specific degradation of double-stranded DNA Fast 2 min protocol for gDNA removal prior reverse transcription Heat-labile - irreversibly inactivated by moderate heat treatment (55°C) Applications Genomic DNA removal from RNA samples prior first strand cDNA synthesis, RT-PCR and RT-qP