Only two primers are required for each target, making the design of multiplex assays much easier The master mix format provides everything you need in one tube
Combine with your template and primers
Works on most currently available real-time instruments capable of measuring more than one fluor
• MgCl2, 1 x 750 μL• GoScript™ 5X Reaction Buffer, 1 x 300 μL• GoScript™ Reverse Transcriptase, 1 x 50 μL• oligo(dT)15 primer, 1 x 50 μg• Nuclease-free Water, 1 x 200μLStore at –30° to –10°C.
• MgCl2, 1 x 1.2 mL• GoScript™ 5X Reaction Buffer, 1 x 600 μL• GoScript™ Reverse Transcriptase, 1 x 100 μL• oligo(dT)15 primer, 1 x 50 μg• Nuclease-free Water, 1 x 200μLStore at –30° to –10°C.