Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Description

• The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications
• It provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure
• These cDNA Synthesis kits use Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT
• The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT
• The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 μg) at elevated temperatures (42–65°C)
• The synthesis reaction can be completed in 15–30 minutes
• Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors
• The Maxima First Strand cDNA Synthesis Kit for RT-qPCR with dsDNase also contains a novel double-strand specific DNase engineered to remove contaminating genomic DNA from RNA preps in two minutes without damage to quality or quantity of RNA
• Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription
• Features of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR include: High yields of full length cDNA up to 20 kb Efficient cDNA synthesis at wide temperature range (42–65°C) Increased synthesis rate—complete cDNA synthesis in 15–30 minutes High sensitivity and specificity Integrated gDNA removal step with dsDNase kit Maxima First Strand cDNA Synthesis Kit with dsDNase contains: Maxima Enzyme Mix 5X Reaction Mix nuclease-free water Kit with dsDNase also contains dsDNase 10X dsDNase Buffer Applications 2-step RT-PCR 2-step RT-qPCR Additional information about reaction components The Maxima Enzyme Mix contains Maxima Reverse Transcriptase and RiboLock RNase Inhibitor
• The recombinant RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B, and C at temperatures up to 55°C
• 5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers
• Nuclease-free water is provided for reaction set-up and dilution of sample RNA
• The absence of endo- and exo-deoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.