FERM1681
Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERM1681-Each-of-1 | In Stock | ₹ 47,400.24 |
FERM1681 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,400.24
GST (18%): ₹ 8,532.043
Total Price: ₹ 55,932.283
Content And Storage
• Maxima H Minus cDNA Synthesis Master Mix (1 x 200 μL)• Maxima H Minus cDNA Synthesis Master Mix 'No RT' Control (1 x 200 μL)• dsDNase (1 x 50 μL)• 10X dsDNase buffer (1 x 100 μL)• Nuclease-free water (1 x 1.25 mL)Store at –5 to –30°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
Maxima H Minus
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Premixed Components
Size (Final Product)
Up to 20 kb
Includes
Kit with dsDNase
Format
Master Mix
Reaction Speed
30 min.
Optimal Reaction Temperature
50°C
Ribonuclease H Activity
Reduced
For Use With (Application)
Real Time PCR (qPCR), RT-PCR
No. of Reactions
50 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Related Products
Description
- Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix
- It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications and reproducible cDNA synthesis at elevated temperatures (50–65°C) within 30 minutes
- The master mix contains Maxima H Minus Reverse Transcriptase (RT) and RiboLock RNase Inhibitor
- Maxima H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution
- The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity
- The recombinant RiboLock RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55°C
- The master mix also contains reaction buffer, dNTPs, oligo (dT) 18 , and random hexamer primers
- A separate tube of No-RT control mix is also provided for convienient RT negative control
- Both kits are capable of reproducible cDNA synthesis at elevated temperatures (50°C–65°C)
- The synthesis reaction is typically complete in 15–30 minutes
- The included double-strand specific DNase in the Maxima H Minus cDNA Synthesis Master Mix with dsDNase allows removal of genomic DNA from RNA samples in two minutes without affecting the quality or quantity of RNA
- This dsDNase is also available separately (Cat
- No
- EN0771)
- Features of Maxima H Minus cDNA Synthesis Master Mix include: • One-tube master mix helps reduce pipetting steps and enhanced consistency in RT-qPCR results • Increased RT efficiency across a wide range of input RNA amounts and gene targets • High thermostability to allow RT reactions at 50–65°C temperature range • Integrated step of genomic DNA removal from RNA samples (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only) Additional information about reaction components • The No RT control mix contains all the components in the Maxima H Minus cDNA Synthesis Master Mix except RT
- The presence of a PCR product in the No RT control reaction indicates that the reaction is contaminated with DNA
- To further enhance genomic DNA elimination efficiency, template RNA incubation with dsDNase (Cat
- No
- EN0771) is strongly recommended
- • Nuclease-free water is provided for reaction setup and dilution of sample DNA
- The absence of exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests
- • To further enhance genomic DNA elimination efficiency, template RNA incubation with the included dsDNase is strongly recommended
- (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only) • The dsDNase is used for rapid and safe removal of contaminating genomic DNA from RNA samples
- dsDNase is easily inactivated by moderate heat treatment (55°C)
- It allows for a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure
- (Maxima H Minus cDNA Synthesis Master Mix with dsDNase only)
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Content And Storage: • Maxima H Minus cDNA Synthesis Master Mix (1 x 200 μL)• Maxima H Minus cDNA Synthesis Master Mix 'No RT' Control (1 x 200 μL)• dsDNase (1 x 50 μL)• 10X dsDNase buffer (1 x 100 μL)• Nuclease-free water (1 x 1.25 mL)Store at –5 to –30°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: Maxima H Minus
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Premixed Components
Size (Final Product): Up to 20 kb
Includes: Kit with dsDNase
Format: Master Mix
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR), RT-PCR
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• Maxima H Minus cDNA Synthesis Master Mix (1 x 200 μL)• Maxima H Minus cDNA Synthesis Master Mix 'No RT' Control (1 x 200 μL)• dsDNase (1 x 50 μL)• 10X dsDNase buffer (1 x 100 μL)• Nuclease-free water (1 x 1.25 mL)Store at –5 to –30°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
Maxima H Minus
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Premixed Components
Size (Final Product):
Up to 20 kb
Includes:
Kit with dsDNase
Format:
Master Mix
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR), RT-PCR
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: • Maxima H Minus cDNA Synthesis Master Mix (1 x 800 μL)• Maxima H Minus cDNA Synthesis Master Mix 'No RT' Control (1 x 200 μL)• dsDNase (1 x 200 μL)• 10X dsDNase buffer (1 x 400 μL)• Nuclease-free water (1 x 1.25 mL)Store at –5 to –30°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: Maxima H Minus
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Premixed Components
Size (Final Product): Up to 20 kb
Includes: Kit with dsDNase
Format: Master Mix
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR), RT-PCR
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• Maxima H Minus cDNA Synthesis Master Mix (1 x 800 μL)• Maxima H Minus cDNA Synthesis Master Mix 'No RT' Control (1 x 200 μL)• dsDNase (1 x 200 μL)• 10X dsDNase buffer (1 x 400 μL)• Nuclease-free water (1 x 1.25 mL)Store at –5 to –30°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
Maxima H Minus
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Premixed Components
Size (Final Product):
Up to 20 kb
Includes:
Kit with dsDNase
Format:
Master Mix
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR), RT-PCR
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: 4° C
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Includes: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Content And Storage:
4° C
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Includes:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Includes: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Includes:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__