11-766-500
Invitrogen™ SuperScript™ IV VILO™ Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-766-500-Each-of-1 | In Stock | ₹ 5,17,980.00 |
11-766-500 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 5,17,980.00
GST (18%): ₹ 93,236.40
Total Price: ₹ 6,11,216.40
Content And Storage
• SuperScript IV VILO Master Mix (2 x 1 mL)• SuperScript IV VILO Master Mix 'No RT' Control (1 mL)• ezDNase Enzyme (500 μL)• 10X ezDNase Buffer (1 mL)• Nuclease-free water (1.25 mL)Store at -15°C to -25°C.
Format
Master Mix
Reaction Speed
10 min.
Optimal Reaction Temperature
50°C
Ribonuclease H Activity
Reduced
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
Reaction Format
Master Mix
Size (Final Product)
Up to 10 kb
Includes
Kit with ezDnase
Detection Method
qPCR
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript IV
Shipping Condition
Dry Ice
Concentration
5X
No. of Reactions
500 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
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Description
- SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications
- The inclusion of ezDNase enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step
- SuperScript IV VILO Master Mix features include: • Fast RT reactions in just 10 minutes • Convenient one-tube cDNA reaction master mix for two-step RT-qPCR • High yields reduce Ct values by more than two cycles ahead of all other reverse transcription reagents • Efficient reaction even with low template amounts and suboptimal purity samples • gDNA removal in as few as two minutes (SSIV VILO Master Mix with ezDNase only) SSIV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV Reverse Transcriptase and further optimized buffer
- These components enable efficient cDNA synthesis at higher temperatures and in less time
- SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates
- It is your new tool for more efficient and reproducible RT-qPCR
- SuperScript IV VILO Master Mix with ezDNase is available for better removal of genomic DNA and to further accelerate the RT-qPCR workflow
- The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment
- SSIV VILO master mix results in more cDNA in less time with less pipetting, less variation, less reaction inhibition, less gDNA interference, and with less sample than other cDNA synthesis kits or master mixes for RT-qPCR
- Additional ezDNase may also be purchased separately
- Source Purified from E
- coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity Performance and quality testing Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product
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Content And Storage: • SuperScript IV VILO Master Mix (2 x 1 mL)• SuperScript IV VILO Master Mix 'No RT' Control (1 mL)• ezDNase Enzyme (500 μL)• 10X ezDNase Buffer (1 mL)• Nuclease-free water (1.25 mL)Store at -15°C to -25°C.
Format: Master Mix
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Size (Final Product): Up to 10 kb
Includes: Kit with ezDnase
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Concentration: 5X
No. of Reactions: 500 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• SuperScript IV VILO Master Mix (2 x 1 mL)• SuperScript IV VILO Master Mix 'No RT' Control (1 mL)• ezDNase Enzyme (500 μL)• 10X ezDNase Buffer (1 mL)• Nuclease-free water (1.25 mL)Store at -15°C to -25°C.
Format:
Master Mix
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Size (Final Product):
Up to 10 kb
Includes:
Kit with ezDnase
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Concentration:
5X
No. of Reactions:
500 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Includes: __
Detection Method: __
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Content And Storage:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Includes:
__
Detection Method:
__
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Concentration:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Content And Storage: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Includes: __
Detection Method: __
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Content And Storage:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Includes:
__
Detection Method:
__
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Concentration:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Content And Storage: EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX in a separate tube.Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format: Tube
Reaction Speed: Fast or Standard
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
For Use With (Application): __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Includes: __
Detection Method: SYBR
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: __
Concentration: __
No. of Reactions: 2500 Reactions
Reagent Type: __
Starting Material: __
Content And Storage:
EXPRESS One-Step SYBR™ GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR™ GreenER™ RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR™ GreenER™ qPCR SuperMix with ROX in a separate tube.Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR™ GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
Format:
Tube
Reaction Speed:
Fast or Standard
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
For Use With (Application):
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Includes:
__
Detection Method:
SYBR
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
__
Concentration:
__
No. of Reactions:
2500 Reactions
Reagent Type:
__
Starting Material:
__