Fractogel® EMD TMAE Hicap (M)

Description

• General description: 8 x 100mm
• Features and Benefits: MiniChrom Column Fractogel® TMAE Hicap (M), 5 ml enables users to:Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughputReduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitizationCompatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solutionDue to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).The strength of the binding depends on the following:the buffer systempH value of the buffer which determines the surface charge of the proteinthe concentration of the counter ionsthe charge density on the support (protein binding capacity)
• Legal Information: FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany