Thermo Scientific™ Imject™ Blue Carrier™ Protein

Description

• Description Thermo Scientific™ Blue Carrier Protein is a highly-soluble, mollusk-derived hemocyanin formulated in convenient package sizes and a complete kit for carbodiimide (EDC-mediated) hapten-carrier conjugation via amine and carboxyl groups
• Blue Carrier Protein is a purified preparation of Concholepas concholepas hemocyanin (CCH)
• The large protein exhibits most of the same immunogenic properties as the popular carrier protein, keyhole limpet hemocyanin (KLH)
• However, its signficantly better solubility provides greater flexibility in immunogen preparation protocols by allowing a broader range of buffer and pH conditions for coupling peptides, proteins and other haptens using classical crosslinkers such as glutaraldehyde, Sulfo-SMCC and EDC
• Highlights: Convenient packages – choose lyophilized Blue Carrier Protein or complete EDC conjugation kit High-yield conjugation – each molecule of CCH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers Validated quality – the immunogenic carrier protein is specially purified to ensure uniform chemical structure and solubility for consistent performance Superior solubility – increased solubility compared to KLH allows Blue Carrier Protein to be used in a broader range of conjugation protocols to accommodate unusual or poorly soluble haptens Highly immunogenic – provides essentially the same level of immunogenicity and phylogenetic distance as keyhole limpet hemocyanin (KLH) Properties of Blue Carrier Protein: Alternative names: Concholepas concholepas hemocyanin, CCH Molecular weight: Whole molecule: ∽8,000,000; Subunits: ∽400,000 and ∽350,000 Absorption: maxima 280nm, 350nm and 558nm Extinction coefficient (Abs
• 1mg/mL solution): 1.4 at 280nm; 0.35 at 346nm A280/A350 Ratio: ∽4.0 Copper/protein ratio: ∽16 Cu 2+ /protein molecule Isoelectric point: 6.0 Solubility: Soluble in aqueous buffers pH 7.0 to 8.0 Stability: Stable in aqueous buffers (∽pH 7.2 without Ca 2+ or Mg 2+ ) Includes: Kit contains lyophilized Blue Carrier Protein (in MES buffer), EDC crosslinker, buffers and desalting columns to easily prepare ready-to-use immunogens for injection