Description

• 5-Ph-IAA is a selective and potent "Bump & Hole" TAG Degrader (DC50,6h = 17.0 nM, T1/2 = 62.3 min), for use within an auxin-inducible degron (AID2) system
• 5-Ph-IAA is an analog (the “Bump”) of natural auxin that induces rapid degradation of a target protein tagged with a single-point mutant OsTIR1F74G F-box protein (the "Hole" or mini AID)
• The induced degradation is reversible 3 h after washout with fresh medium
• 5-Ph-IAA shows rapid depletion of mAID-tagged nuclear and cytoplasmic proteins in proliferating and non-proliferating mammalian cells with no detectable basal degradation, lower cytotoxicity level, increased potency and faster degradation of the reporter in comparison with the conventional AID system
• The AID2 system also works in budding yeast and fission yeast, and it can control protein expression in multiple mouse organs in vivo
• To enquire about custom knock-in cell lines for the AID2 platform, please email TPD@bio-techne.com Plasmid vectors for the AAV transfection and CRISPR-mediated knock-in of OsTIR1F74G and mAID tag are available from Addgene .