Invitrogen™ TOP10 Electrocomp™ Kit

Description

• TOP10 Electrocomp E
• coli cells are provided at a transformation efficiency of 1 x 10 10 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation
• This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids
• The TOP10 genotype is similar to the DH10B strain and features: • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones • end A1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I • rec A1 for reduced occurrence of non-specific recombination in cloned DNA Notes Please see the product manual for reaction scale recommendations
• A high-voltage electroporation apparatus is required
• Genotype F - mcr A Δ( mrr - hsd RMS- mcr BC ) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara - leu )7697 gal U gal K rps L end A1 nup G Find the strain and format that fits your needs • TOP10 is available in chemically competent and electrocompetent cell formats
• • Choose MultiShot competent cells for high-throughput cloning applications.