Invitrogen™ One Shot™ TOP10F' Chemically CompetentE. coli

Description

• One Shot TOP10F´ Chemically Competent E
• coli are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries a tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication
• In addition, the F´ episome carries the lac Iq repressor for inducible expression from trc , tac , and lac promoters using IPTG
• TOP10F´ competent cells are provided at a transformation efficiency of >1 x 10 9 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation
• As a derivative of TOP10, TOP10F´ Chemically Competent E
• coli cells carry mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ) allowing them to clone both prokaryotic and eukaryotic genomic DNA and provide efficient plasmid rescue from eukaryotic genomes
• TOP10F´ cells also carry rec A1 and end A1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps
• One Shot TOP10F´ Chemically Competent E
• coli cells offer: • Transformation efficiencies of >1 x 10 9 cfu/μg • Stable F´ episome that allows isolation of ssDNA and carries lac Iq repressor for inducible expression from trc , tac , and lac promoters • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones on plates containing X-Gal or Bluo-Gal • end A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA Note: IPTG induction is required for blue-white color screening
• Easy-to-use One Shot format TOP10 F´ Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and preventing contamination
• Genotype F´{ lac Iq, Tn 10(TetR)} mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara-leu )7697 gal U gal K rps L(StrR) end A1 nup G Find the strains and formats that fit your needs • DH strains are available in chemically competent and electrocompetent cell formats
• • For high throughput applications there are a variety of TOP10 strains in MultiShot formats
• • For ssDNA production try strains such as TOP10F´ (Cat
• No
• C303003) or electrocompetent DH12S (Cat
• No
• 18312017)
• Order Info Shipping Condition: Dry Ice