Invitrogen™ One Shot™ OmniMAX™ 2 T1RChemically CompetentE. coli

Description

• Description The One Shot OmniMAX 2 T1 R E
• coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 10 9 transformants/μg pUC19 DNA
• It offers the highest transformation efficiency of any chemically competent cells in a One Shot format
• OmniMAX 2 T1 R is a versatile strain, perfect to use in all cloning applications, including Gateway technology and TOPO PCR cloning
• OmniMAX 2 T1 R Chemically Competent E
• coli cells carry the ton A (also known as fhu A) mutation, which confers resistance to T1 and T5 phage infection
• The ton A genotype offers added security to valuable clones and libraries
• This strain lacks the E
• coli K12 restriction systems ( mcr A Δ( mrr hsd RMS- mcr BC)), allowing efficient transformation of highly methylated DNA
• The OmniMAX 2 T1 R strain has the lac ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal
• It also carries the rec A1 mutation, which helps reduce the rate of recombination while propagating plasmid DNA, and the end A1 mutation that increases plasmid DNA yield and quantity
• The OmniMAX 2 T1 R strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids)
• In addition, the F´ episome carries the lac Iq repressor for inducible expression from trc , tac , and lac promoters using IPTG
• Importantly, the ccdA gene present on the F´ episome is deleted, allowing negative selection of Gateway vectors as the strain is sensitive to the ccd B gene product
• One Shot OmniMAX 2 T1 R Chemically Competent E
• coli offer: • Highest transformation efficiencies of >5 x 10 9 cfu/μg • ton A ( fhu A) genotype to confer resistance to T1 and T5 phage • Δ( ccd AB) for sensitivity to the toxic effects of the ccd B gene product, allowing negative selection of vectors containing the ccd B gene (Gateway cloning system) • Stable F´ episome that carries lac Iq repressor for inducible expression from trc , tac , and lac promoters • Construction of more representative genomic libraries due to the elimination of mcr A, mcr BC, mrr , and hsd RMS • lac ZΔM15 for blue-white color screening of recombinant clones • end A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA Easy-to-use One Shot format OmniMAX 2 T1 R Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination
• Genotype F´ [ pro AB+ lac Iq lac ZΔM15 Tn 10(TetR) Δ( ccd AB)] mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 end A1 rec A1 sup E44 thi -1 gyr A96 (NalR) rel A1 ton A pan D Find the strain and format that fit your needs • We offer other strains in chemically competent and electrocompetent cell formats to meet your specific needs
• • OmniMAX 2 T1 R and other strains are available in MultiShot formats for high throughput applications
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