Invitrogen™ One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

Description

• Description One Shot MAX Efficiency DH10B T1 Phage-Resistant E
• coli have all the benefits of the DH10B genotype, with the addition of the ton A (also known as fhuA ) genotype for resistance to T1 and T5 phage infection
• The inclusion of the ton A genotype offers added security to protect valuable clones and libraries
• MAX Efficiency DH10B T1 cells can achieve high transformation efficiency of >1 x 109 cfu/μg supercoiled plasmid, making them ideal for generating cDNA or genomic libraries, transforming large plasmids (>150 kb in size), and for situations when DNA is limiting
• MAX Efficiency DH10B T1 Phage-Resistant Cells carry mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ), allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes
• This strain also has the lac ZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal
• Finally, the end A1 mutation makes DH10B-T1R cells an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification
• MAX Efficiency DH10B T1 Phage-Resistant Cells offer: • Transformation efficiencies of >1 x 10 9 cfu/μg • ton A ( fhuA ) genotype to confer resistance to T1 and T5 phage • lac ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal • mcr A genotypic marker and the mcr BC, mrr deletion that allows for cloning DNA that contains methylcytosine and methyladenine • end A1 mutation that enables increased plasmid yield and quantity Convenient and efficient format DH10B-T1R E
• coli are supplied in the convenient, single-reaction One Shot format
• Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles, or money wasted on unused cells
• Genotype F – mcr A Δ( mrr - hsd RMS- mcr BC) Φ80 lac ZΔM15 Δ lac X74 rec A1 end A1 ara D139 Δ( ara , leu )7697 gal U gal K λ- rps L nup G ton A Find the strain and format that fit your needs • We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs
• • The DH10B-T1R strain is available in Multishot format for high throughput applications.