C404006
Invitrogen™ One Shot™ TOP10 Chemically CompetentE. coli
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | C404006-Each-of-1 | In Stock | ₹ 77,688.48 |
C404006 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 77,688.48
GST (18%): ₹ 13,983.926
Total Price: ₹ 91,672.406
Product Type
Chemically Competent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Blue/White Screening
Yes (lacZΔM15)
Plasmid
High Copy Plasmid
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
No
Format
Tube
Quantity
42 x 50 μL
Contains F' Episome
No
Cloning Methylated DNA
Yes (mcrA)
Content And Storage
• One Shot TOP10 Chemically CompetentE. coli(42 x 50 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA
No
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
One Shot™
Related Products
Description
- Description One Shot TOP10 Chemically Competent E
- coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 10 9 cfu/μg plasmid DNA
- These cells allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits
- TOP10 E
- coli cells are genetically similar to the DH10B strain and have been reported to be more resilient to stress conditions like osmotic shock and acidic pH stress
- One Shot TOP10 cells: • Maximize cloning efficiency in a single-tube format • Provide enhanced genomic DNA cloning capabilities TOP10 Chemically Competent E
- coli cells carry mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ) allowing the cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes
- Similar to other DH strains, TOP10 has the lac ZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal
- The inclusion of rec A1 and end A1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps
- One Shot TOP10 Chemically Competent E
- coli cells offer: • Transformation efficiencies of >1 x 10 9 cfu/μg • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones • end A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA • Expression from the lac promoter without IPTG Easy-to-use One Shot format TOP10 Chemically Competent E
- coli cells are supplied in the convenient, single-reaction One Shot format
- The single-tube, single-use format accomodates allows all steps of the transformation protocol, up to plating, to take place in the same tube, helping save time and prevent contamination
- Genotype F – mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara-leu )7697 gal U gal K λ– rps L(Str R ) end A1 nup G Find the strain and format that fit your needs • We offer other DH strains in chemically competent and electrocompetent cell formats
- • The TOP10 strain is available in several MultiShot formats for high throughput applications
- Order Info Shipping Condition: Dry Ice
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Show Difference
Product Type: Chemically Competent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Plasmid: High Copy Plasmid
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 42 x 50 μL
Contains F' Episome: No
Cloning Methylated DNA: Yes (mcrA)
Content And Storage: • One Shot TOP10 Chemically CompetentE. coli(42 x 50 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA: No
High-throughput Compatibility: Low
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: One Shot™
Product Type:
Chemically Competent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial:
Yes (Streptomycin)
Blue/White Screening:
Yes (lacZΔM15)
Plasmid:
High Copy Plasmid
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
42 x 50 μL
Contains F' Episome:
No
Cloning Methylated DNA:
Yes (mcrA)
Content And Storage:
• One Shot TOP10 Chemically CompetentE. coli(42 x 50 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA:
No
High-throughput Compatibility:
Low
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
One Shot™
Product Type: Chemically Competent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Plasmid: High Copy Plasmid
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 11 x 50 μL
Contains F' Episome: No
Cloning Methylated DNA: Yes (mcrA)
Content And Storage: • One Shot TOP10 Chemically CompetentE. coli(11 x 50 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA: No
High-throughput Compatibility: Low
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: One Shot™
Product Type:
Chemically Competent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial:
Yes (Streptomycin)
Blue/White Screening:
Yes (lacZΔM15)
Plasmid:
High Copy Plasmid
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
11 x 50 μL
Contains F' Episome:
No
Cloning Methylated DNA:
Yes (mcrA)
Content And Storage:
• One Shot TOP10 Chemically CompetentE. coli(11 x 50 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA:
No
High-throughput Compatibility:
Low
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
One Shot™
Product Type: __
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Antibiotic Resistance Bacterial: __
Blue/White Screening: __
Plasmid: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: __
Quantity: __
Contains F' Episome: __
Cloning Methylated DNA: __
Content And Storage: __
Cloning Unstable DNA: __
High-throughput Compatibility: __
Preparing Unmethylated DNA: __
Shipping Condition: __
Species: __
Product Line: __
Product Type:
__
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Antibiotic Resistance Bacterial:
__
Blue/White Screening:
__
Plasmid:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
__
Quantity:
__
Contains F' Episome:
__
Cloning Methylated DNA:
__
Content And Storage:
__
Cloning Unstable DNA:
__
High-throughput Compatibility:
__
Preparing Unmethylated DNA:
__
Shipping Condition:
__
Species:
__
Product Line:
__
Product Type: Electrocompetent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Plasmid: May be used for plasmids >20 kb
Reduces Recombination: Yes (recA13)
T1 Phage - Resistant (tonA): Yes (Streptomycin)
Format: Tube
Quantity: 11 x 50 μL
Contains F' Episome: No
Cloning Methylated DNA: Yes (mcrA)
Content And Storage: • One Shot TOP10 ElectrocompE. coli(11 x 50 μL)Store at –80°C.• pUC19 DNA (50 μL)Store at –20°C.• S.O.C. Medium (6 mL)Store at room temperature.
Cloning Unstable DNA: No
High-throughput Compatibility: Low
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: One Shot™
Product Type:
Electrocompetent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial:
No
Blue/White Screening:
Yes (lacZΔM15)
Plasmid:
May be used for plasmids >20 kb
Reduces Recombination:
Yes (recA13)
T1 Phage - Resistant (tonA):
Yes (Streptomycin)
Format:
Tube
Quantity:
11 x 50 μL
Contains F' Episome:
No
Cloning Methylated DNA:
Yes (mcrA)
Content And Storage:
• One Shot TOP10 ElectrocompE. coli(11 x 50 μL)Store at –80°C.• pUC19 DNA (50 μL)Store at –20°C.• S.O.C. Medium (6 mL)Store at room temperature.
Cloning Unstable DNA:
No
High-throughput Compatibility:
Low
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
One Shot™