Invitrogen™ MultiShot™ StripWell TOP10 Chemically CompetentE. coli

Description

• MultiShot StripWell TOP10 Chemically Competent E
• coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 10 8 cfu/μg with control plasmid DNA in StripWell format
• These cloning competent cells are packaged in a rack containing 12 strips of 8 tubes to increase productivity for throughput bacterial transformations
• Versatile TOP10 cloning capabilities MultiShot StripWell TOP10 Chemically Competent E
• coli cells are the laboratory workhorse for chemical transformations as they are compatible with a broad spectrum of DNA types for transformation
• They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits
• TOP10 E
• coli cells are genetically similar to the DH10B strain and have been reported to be more resilient to stress conditions (osmotic shock and acidic pH stress)
• These cells carry mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ), allowing cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes
• Similar to other DH strains, TOP10 cells contain rec A1 and end A1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps
• MultiShot StripWell TOP10 Chemically Competent E
• coli cells offer: • Transformation efficiencies of >1 x 10 8 cfu/μg • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal • end A1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I • rec A1 for reduced occurrence of non-specific recombination in cloned DNA Note: TOP10 cells do not require IPTG to induce expression from the lac promoter
• MultiShot StripWell flexible format Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes
• This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and reducing chance of contamination
• After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube
• StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots and avoiding freeze-thaws that can result in reduced transformation efficiency
• Benefits of the MultiShot StripWell format include: • Increased productivity —suitable for high throughput experimentation • Maximal flexibility —transform as few as 1 tube or as many as 96 from the StripWell rack • Familiar convenience —add DNA directly to cells, heat shock, and recover in the same tube Genotype F – mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara-leu )7697 gal U gal K λ– rps L(Str R ) end A1 nup G Find the strain and format that fits your needs • Many other E
• coli strains and formats of chemically competent and electrocompetent cells are also available to meet specific transformation needs
• • For high-throughput transformation, choose from our collection of MultiShot formatted comp cells
• • We also offer bacterial growth media in various formats
• Order Info Shipping Condition: Dry Ice.