Invitrogen™ MultiShot™ TOP10 Chemically CompetentE. coli

Description

• MultiShot TOP10 Chemically Competent E
• coli cells are derived from DH10B and are one of the most popular laboratory strains
• Packaged in five 96-well microtiter plates (15 μL aliquots) to simplify high-throughput workflows and provide transformation efficiencies of >1 x 10 8 cfu/μg control plasmid DNA, Multishot TOP10 is a versatile strain that can be used in multiple routine cloning applications
• Benefits of MultiShot TOP10 competent cells • TOP10 strain is ideal for high-efficiency cloning and plasmid propagation • Microtiter plate format design is convenient for automated high-throughput cloning • mcr A mutation supports efficient transfection of methylated DNA • l ac ZΔM15 allele permits rapid blue/white screening of transfectants • end A1 mutation reduce endonuclease activity and improve plasmid DNA yields • rec A1 mutation helps reduce unwanted recombination Easy to use for high-throughput transformations MultiShot TOP10 Chemically Competent cells in 96-well plates are designed to facilitate high-throughput transformation
• This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination
• After the addition of DNA, MultiShot TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate
• Using our TA Cloning and TOPO Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected
• Genotype F - mcr A Δ( mrr - hsd RMS- mcr BC) Φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara , leu )7697 gal U gal K rps L (StrR) end A1 nup G Find the strain and format that fits your needs • DH strains are available in chemically competent and electrocompetent cell formats
• • For high-throughput transformation options, choose from our collection of MultiShot formatted comp cells
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