Invitrogen™ One Shot™ INVαF' Chemically CompetentE. coli

Description

• One Shot INVαF´ Chemically Competent E
• coli are designed to stably replicate high-copy number plasmids
• They allow for the preparation of high quality plasmid DNA with less carry over contaminants as compared to other popular strains such as JM109 and can achieve a transformation efficiency of >1 x 10 8 cfu/μg supercoiled plasmid
• INVαF´ cells carry the F´ episome and so can be utilized to produce single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication
• This strain is related to DH strains and features similar genetic traits and benefits
• The lac ZΔM15 marker provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and can be used for blue-white screening of colonies on bacterial plates containing Bluo-Gal or X-Gal
• In addition to supporting blue/white screening, rec A1 and end A1 mutations increase insert stability and improve the quality of plasmid DNA
• One Shot INVαF´ Chemically Competent E
• coli cells offer: • Transformation efficiencies of >1 x 10 8 cfu/μg • Stability and exceptional quality of high-copy number plasmid DNAb • Blue-white screening of recombinant clones ( lac ZΔM15) • Reduction of homologous recombination of transformed plasmids ( rec A1) • Increased quality of plasmid DNA preparations ( end A1) • Production and rescue of ssDNA from vectors carrying the f1 origin of replication (F´) Note: INVαF´ cells do not require IPTG to induce expression from the lac promoter
• Convenient and efficient format One Shot INVαF´ Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• Each tube contains enough cells for one complete transformation, reducing freeze-thaw cycles and limiting money wasted on unused cells
• Genotype F´ end A1 rec A1 hsd R17 (r K – , m K + ) sup E44 thi -1 gyr A96 rel A1 φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 λ – Genetic marker descriptions Find the strain and format that fit your needs • A variety of DH strains are available in chemically competent and electrocompetent cell formats
• • We offer other strains for ssDNA production, such as TOP10F´ (Cat
• No
• C303003) or electrocompetent DH12S (Cat
• No
• 18312017)
• • MultiShot formats are available for high throughput applications.