Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1RCompetent Cells

Description

• One Shot MAX Efficiency DH5α-T1 R Competent Cells are derived from the popular DH5α strain and carry the ton A (f huA ) mutation that confers resistance to T1 and T5 phage
• They offer added security to protect valuable clones and libraries
• This is especially important for genome and sequencing centers where T1 phage infection would be detrimental
• DH5α-T1 R is a versatile strain that can be used in many everyday cloning applications
• Prepared by a proprietary modification of the Hanahan method, these cells are suitable for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors
• The lacZ ∆M15 marker provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and therefore can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal
• DH5α-T1 R Competent Cells are capable of being transformed efficiently with large plasmids and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF'IQ is provided to allow plaque formation
• Benefits of MAX Efficiency DH5α-T1 R Competent Cells • Transformation efficiency of >1 x 10 9 transformants/μg with non-saturating amounts of DNA • ton A ( fhuA ) mutation to confer resistance to T1 and T5 phage • end A1 mutation that enables cleaner DNA preparations and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I • rec A1 mutation that reduces occurrence of unwanted recombination in cloned DNA • hsdR for efficient transformation of unmethylated DNA from PCR applications • lac ZΔM15 marker that allows blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase Convenient and efficient format DH5α-T1 R E
• coli are supplied in the convenient, single-reaction One Shot format
• Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells
• Genotype: F - φ80 lac ZΔM15 Δ(lacZYA- arg F)U169 rec A1 end A1 hsd R17(rK-, mK+) pho A sup E44 thi -1 gyr A96 rel A1 ton A Find the strain and format that fits you needs • DH strains are available in chemically competent and electrocompetent cell formats
• • MAX Efficiency DH5αF´IQ Competent Cells (Cat
• No
• 18288019) are available in chemically competent format
• • DH5α-T1 R is available in Multishot format for high throughput applications
• Order Info Shipping Condition: Dry Ice