Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically CompetentE. coli

Description

• Description One Shot Mach1 T1 Phage-Resistant Chemically Competent E
• coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes
• Mach1 T1 R colonies are clearly visible within eight hours of plating the transformation mix (ampicillin selection), enabling you to plate and pick colonies the same day
• Plasmid DNA can be prepared four hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic)
• Mach1 T1 R Chemically Competent E
• coli cells can achieve transformation efficiency of >1 x 10 9 cfu/μg plasmid DNA
• The Mach1 T1 R strain is derived from a non-K-12, wild-type W E
• coli strain (ATCC #9637) that had been selected due it’s growth speed and genetically manipulated to possess the most useful cloning strain features
• The strain was engineered to carry the ton A (also known as fhu A) mutation that confers resistance to T1 and T5 phage infection
• The ton A genotype offers added security to valuable clones and libraries
• The Mach1 T1 R strain has the lac ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal
• It also carries the rec A1398 mutation that helps reduce the rate of recombination while propagating plasmid DNA, the end A1 mutation that increases plasmid DNA yield and quantity, and hsd R restriction system deletion to allow genomic DNA cloning
• One Shot Mach1 T1 Phage-Resistant Chemically Competent E
• coli offer: • Transformation efficiencies of >1 x 10 9 cfu/μg plasmid DNA • ton A ( fhu A) genotype to confer resistance to T1 and T5 phage • lac Z∆M15 for blue-white color screening of recombinants • hsd R mutation for efficient transformation of unmethylated DNA from PCR applications • rec A1398 mutation for reduced occurrence of homologous recombination in cloned DNA • end A1 mutation for increased plasmid yield and quality Easy-to-use One Shot format Mach1 T1 Phage-Resistant Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, helping save time and prevent contamination
• Genotype F – φ80 lac ZΔM15 Δ lac X74 hsd R(r K –, m K +) Δ rec A1398 end A1 ton A Find the strain and format that you need • We offer other strains in chemically competent and electrocompetent cell formats to meet your specific needs
• • Mach1 T1 R and other strains are available in MultiShot formats for high throughput applications.