Invitrogen™ MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically CompetentE. coli

Description

• MultiShot StripWell Mach1 T1 R chemically competent E
• coli cells are exceptionally fast-growing cloning competent cells packaged in a rack containing 12 strips of 8 tubes for increased productivity of bacterial transformations
• The Mach1 T1 R strain is derived from a non-K-12, wild-type W E
• coli strain (ATCC #9637) that was selected for its rapid growth rate, and genetically manipulated to possess the most useful cloning strain features
• All of our T1 R strains have the ton A (also known as fhu A) deletion in their genotype that confers resistance to T1 and T5 phage
• T1 bacteriophage spread rapidly and lyse E
• coli hosts, which are commonly used for cloning and library construction
• A resistant genotype offers added security to protect valuable clones and libraries
• This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic
• Accelerate cloning workflow using Mach1 T1 R strain MultiShot StripWell Mach1 T1 R competent E
• coli cells are fast growing chemically competent cells with a doubling time of approximately 50 minutes, with other popular strains averaging 70 minutes
• Mach1-T1R colonies are clearly visible within 8 hours of plating the transformation mix (ampicillin selection), enabling you to plate and pick colonies the same day
• You can also prepare plasmid DNA 4 hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic)
• The Mach1 T1 R strain is useful for many cloning applications and provides transformation efficiencies in the MultiShot StripWell format of >1 x 10 8 cfu/μg with plasmid DNA
• The Mach1 T1 R strain was engineered to carry the ton A mutation that confers resistance to T1 and T5 phage infection
• The ton A genotype offers added security to valuable clones and libraries
• The Mach1 T1 R strain has the lac ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal
• It also carries the rec A1398 mutation that helps reduce the rate of recombination while propagating plasmid DNA, the end A1 mutation that increases plasmid DNA yield and quantity, and hsd R restriction system deletion to allow genomic DNA cloning
• MultiShot StripWell Mach1 T1 R Competent Cells offer: • Transformation efficiencies in StripWell format of >1 x 10 8 cfu/μg plasmid DNA • ton A ( fhu A) genotype to confer resistance to T1 and T5 phage • lac Z∆M15 for blue-white color screening of recombinants • hsd R mutation for efficient transformation of unmethylated DNA from PCR applications • rec A1398 mutation for reduced occurrence of homologous recombination in cloned DNA • end A1 mutation for increased plasmid yield and quality MultiShot StripWell flexible format Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes
• This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and reducing chance of contamination
• After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube
• The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency
• Benifits of the MultiShot StripWell format include: • Increased productivity —suitable for high throughput experimentation • Maximal flexibility —transform as few as 1 or as many as 96 tubes from the StripWell rack • Familiar convenience —add DNA directly to cells, heat shock, and recover in the same tube Genotype F – φ80 lac ZΔM15 Δ lac X74 hsd R(r K –, m K +) Δ rec A1398 end A1 ton A Find the strain and format that fits your needs • A variety of E
• coli strains and formats of chemically competent and electrocompetent cells are available to meet transformation needs
• • For high-throughput transformation choose from our collection of MultiShot comp cells
• • We offer bacterial growth media in various formats.