Invitrogen™ MultiShot™ FlexPlate Mach1™ T1RCompetent Cells

Description

• MultiShot FlexPlate Mach1 T1 R chemically competent E
• coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity
• These chemically competent cells are well suited to reduce the time required for clone evaluation
• The convenient FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput
• All of our T1 R strains have the ton A (also known as fhu A) deletion in their genotype that confers resistance to T1 and T5 phage
• T1 bacteriophage spread rapidly and lyse E
• coli hosts, which are commonly used for cloning and library construction
• A resistant genotype offers added security to protect valuable clones and libraries
• This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic
• Accelerate cloning workflow using Mach1 T1 R strain MultiShot FlexPlate Mach1 T1 R competent E
• coli cells are fast growing chemically competent cells with a doubling time of approximately 50 minutes
• Colonies are clearly visible within 8 hours of plating the transformation mix (ampicillin selection), enabling for plating and selecting of colonies the same day
• Plasmid DNA may also be prepared in four hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic)
• MultiShot FlexPlate Mach1 T1 R Competent Cells offer: • Transformation efficiencies of >1 x 10 8 cfu/μg plasmid DNA • ton A ( fhu A) genotype to confer resistance to T1 and T5 phage • lac Z∆M15 for blue-white color screening of recombinants • hsd R mutation for efficient transformation of unmethylated DNA from PCR applications • rec A1398 mutation for reduced occurrence of homologous recombination in cloned DNA • end A1 mutation for increased plasmid yield and quality Increase transformation productivity with MultiShot FlexPlate format The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput
• Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system
• After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler
• Key benefits of the MultiShot FlexPlate format: • Increase throughput —compatible with automated liquid handling platforms • Flexible —transform in increments of 8 wells to entire 96-well plate • Convenient —competent cells pre-aliquoted into 96-well plate, ready for transformation Genotype: F – φ80 lac ZΔM15 Δ lac X74 hsd R(r K –, m K +) Δ rec A1398 end A1 ton A Find the strain and format that you need • A variety of E
• coli strains and formats of Chemically Competent cells and Electrocompetent cells are available to meet specific transformation needs
• • We offer bacterial growth media in various formats
• • For high-throughput transformation options, choose from our collection of MultiShot formatted comp cells.