Invitrogen™ MultiShot™ FlexPlate OmniMAX™ 2 T1RCompetent Cells

Description

• MultiShot FlexPlate OmniMAX 2 T1 R chemically competent E
• coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity
• The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput
• All of our T1 R strains have the ton A (also known as fhu A) deletion in their genotype that confers resistance to T1 and T5 phage
• T1 bacteriophage spread rapidly and lyse E
• coli hosts, which are commonly used for cloning and library construction
• A resistant genotype offers added security to protect valuable clones and libraries
• This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic
• Achieve highest transformation efficiency with OmniMAX 2 T1 R chemically competent cells The MultiShot FlexPlate OmniMAX 2 T1 R E
• coli strain is an improved chemically competent cell line that provides transformation efficiencies in the MultiShot FlexPlate format of >5 x 10 8 cfu/μg with control plasmid DNA
• It is versatile strain, perfect to use in all cloning applications, including with Gateway technology and TOPO PCR cloning
• The OmniMAX 2 T1 R strain lacks the E
• coli K12 restriction systems ( mcr A Δ( mrr hsd RMS- mcr BC)) allowing efficient transformation of highly methylated DNA
• The OmniMAX 2 T1 R strain has the lac ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal
• It also carries the rec A1 mutation that helps reduce the rate of recombination while propagating plasmid DNA, and the end A1 mutation that increases plasmid DNA yield and quantity
• The OmniMAX 2 T1 R strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids)
• In addition, the F´ episome carries the lac Iq repressor for inducible expression from trc , tac , and lac promoters using IPTG
• Importantly, the ccd A gene present on the F´ episome is deleted, allowing negative selection of Gateway vectors since the strain is sensitive to the ccd B gene product
• One Shot OmniMAX 2 T1 R Chemically Competent E
• coli offer: • Highest transformation efficiency in FlexPlate format of >5x10 8 cfu/μg control DNA • ton A ( fhu A) genotype to confer resistance to T1 and T5 phage • Δ( ccd AB) for sensitivity to toxic effects of ccd B gene product, allowing negative selection of vectors containing ccd B gene (Gateway cloning system) • Stable F´ episome that carries lac Iq repressor for inducible expression from trc , tac , and lac promoters • Construction of more representative genomic libraries due to the elimination of mcr A, mcr BC, mrr , and hsd RMS • lac ZΔM15 for blue-white color screening of recombinant clones • end A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA Increase transformation productivity with MultiShot FlexPlate format The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput
• Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system
• After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler
• Key benefits of the MultiShot FlexPlate format: • Increase throughput —compatible with automated liquid handling platforms • Flexible —transform in increments of 8 wells to entire 96-well plate • Convenient —competent cells pre-aliquoted into 96-well plate, ready for transformation Genotype F´ [ pro AB+ lac Iq lac ZΔM15 Tn 10(TetR) Δ( ccd AB)] mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 end A1 rec A1 sup E44 thi -1 gyr A96 (NalR) rel A1 ton A pan D Find the strain and format that you need • We offer many other E
• coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs
• • If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells
• • We offer bacterial growth media in various formats.