Invitrogen™ MultiShot™ FlexPlate DH10B T1RCompetent Cells

Description

• MultiShot FlexPlate DH10B T1 R chemically competent E
• coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity
• The FlexPlate format can be conveniently divided into 8-well segments for flexibility in transformation throughput
• All of our T1 R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage
• T1 bacteriophage spread rapidly and lyse E
• coli hosts, which are commonly used for cloning and library construction
• A resistant genotype offers added security to protect valuable clones and libraries
• This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic
• Diverse DH10B T1 R cloning capabilities MultiShot FlexPlate DH10B T1R competent cells may be used in a variety of cloning applications as they are resistant to the effects of ligase and ligation buffers and provide transformation efficiencies of >1 x 10 8 cfu/μg with control plasmid DNA
• The cells are ideal for cloning large plasmids and BACs and are suitable for cloning DNA that contains methylcytosine and methyladenine (i.e., genomic DNA)
• MultiShot FlexPlate DH10B T1R Competent Cell features • mcr A, mcr BC, mrr , and hsd RMS elimination of restriction systems to allow construction of more representative genomic constructs • ton A (fhuA) genotype to confer resistance to T1 and T5 phage • end A1 for increased plasmid yield and quantity • rec A1 for reduced occurrence of non-specific recombination in cloned DNA • lac ZΔM15 for blue/white color screening of recombinant clones Increase transformation productivity with MultiShot FlexWell format The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput
• Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system
• After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler
• Key benefits of the MultiShot FlexPlate format include: • Increase throughput—compatible with automated liquid handling platforms • Flexible 96-well plate may be divided into 8-wellsstrips • Competent cells come pre-aliquoted in 96-well plates and ready for transformation Genotype F - mcr A Δ( mrr-hsd RMS- mcr BC) φ80 lac ZΔM15 lac X74 rec A1 end A1 ara D139 Δ( ara-leu )7697 gal U gal K rps L nup G λ- ton A Find the strain and format that you need • We offer many E
• coli strains and formats of chemically competent and electrocompetent cells to meet your specific transformation needs
• • Choose from our MultiShot formatted comp cells for high-throughput options.