Invitrogen™ MultiShot™ FlexPlate DH5α T1RCompetent Cells

Description

• MultiShot FlexPlate DH5α T1 R Chemically Competent E
• coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications
• These cells are pre-aliquoted in a 96-well PCR plate to increase transformation productivity, and the FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput
• All of our T1 R strains have the ton A (also known as fhu A) deletion in their genotype that confers resistance to T1 and T5 phage
• T1 bacteriophage spread rapidly and lyse E
• coli hosts, which are commonly used for cloning and library construction
• A resistant genotype offers added security to protect valuable clones and libraries
• Reliable DH5α T1 R cloning capabilities MultiShot FlexPlate DH5α T1 R competent E
• coli cells are able to achieve transformation efficiencies of >1 x 10 8 cfu/μg control DNA
• This strain is recommended for transformations of cloned unmethylated DNA, routine cloning of genes into large plasmids, and is suitable for difficult clone constructions, such as blunt-end ligations or cloning using limited amount of DNA
• The DH5α T1 R strain carries the hsd R mutation to prevent restriction of foreign DNA and the end A1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols
• The lac ZΔM15 marker provides α-complementation of the ß-galactosidase gene allowing blue-white screening on agar plates containing X-Gal or Bluo-Gal
• MultiShot FlexPlate DH5α T1 R Competent Cells offer: • Transformation efficiency of >1 x 10 8 transformants/μg plasmid DNA • ton A ( fhu A) genotype to confer resistance to T1 and T5 phage • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • lacZ ΔM15 marker for blue-white screening • end A1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I • rec A1 for reduced occurrence of non-specific recombination in cloned DNA Increase transformation productivity with MultiShot FlexPlate format The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput
• Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system
• After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler
• Key benefits of the MultiShot FlexPlate format • Increased throughput —compatible with automated liquid handling platforms • Flexible —transform in increments of 8 wells to entire 96-well plate • Convenient —competent cells pre-aliquoted into 96-well plate, ready for transformation Genotype F – φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 rec A1 end A1 hsd R17 (r K –, m K +) pho A sup E44 λ– thi -1 gyr A96 rel A1 ton A Find the strain and format that fits your needs • Many other E
• coli strains and formats of chemically competent and electrocompetent cells are also available to meet specific transformation needs
• • For high-throughput transformation, choose from our collection of MultiShot formatted comp cells
• • We also offer bacterial growth media in various formats.