Invitrogen™ MultiShot™ StripWell DH5α T1RCompetent Cells

Description

• MultiShot StripWell DH5α T1 R chemically competent E
• coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations
• This strain is derived from the popular DH5α strain, one of the most frequently used laboratory cloning strains
• All of our T1 R strains have the ton A (also known as fhu A) mutation in their genotype that confers resistance to T1 and T5 phages
• A resistant genotype offers added security to protect valuable clones and libraries as T1 bacteriophage can rapidly spread among E
• coli hosts and contaminate repositories
• DH5α T1 R for routine cloning applications The DH5α strain is well-known and broadly used laboratory strain for chemical transformations as it is ideal for transformations of cloned unmethylated DNA
• To increase productivity for medium-throughput bacterial transformations, the cloning competent DH5α T1 R E
• coli cells are packaged in racks containing 12 strips of 8
• MultiShot StripWell DH5α T1 R Competent Cells include: • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • lac ZΔM15 for blue/white color screening of recombinant clones • end A1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I • rec A1 for reduced occurrence of non-specific recombination in cloned DNA • ton A ( fhu A) for T1 and T5 phage resistance MultiShot StripWell flexible format Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes
• This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and reducing chance of contamination
• After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C in a water bath without transfering to a new tube
• The StripWell tubes can also be cut to permit single transformations
• This feature eliminates wasted aliquots and avoids needless freeze-thaw cycles that can result in reduced transformation efficiency
• Benifits of the MultiShot StripWell format include: • Increased productivity—suitable for high throughput experimentation • Maximal flexibility—transform as few as 1 tube or as many as 96 from the StripWell rack • Familiar convenience—add DNA directly to cells, heat shock, and recover in the same tube Genotype F - φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 rec A1 end A1 hsd R17 (rK–, mK+) pho A sup E44 λ thi -1 gyr A96 rel A1 ton A Find the strain and format that you need • We offer a variety of DH strains in both chemically competent and electrocompetent cell formats
• • For high-throughput transformation options, choose from our collection of MultiShot Formatted Competent Cells.