Invitrogen™ MAX Efficiency™ DH5α-T1RCompetent Cells

Description

• MAX Efficiency DH5α T1 R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5
• In addition to supporting blue/white screening, rec A1 and end A1 mutations in DH5α increase insert stability and improve the quality of plasmid DNA prepared from minipreps
• These cells are suitable for the construction of gene banks or generation of cDNA libraries using derived vectors
• MAX Efficiency DH5α T1 R Phage-Resistant Competent Cells have been prepared by a patented modification of the procedure of Hanahan and yield >1 x 10 9 transformants/μg of monomer pUC19 DNA in a 100 μL transformation reaction
• MAX Efficiency DH5α T1 R Phage Resistant Competent Cell features • Resistance to the lytic bacteriophage T1, one of the most dangerous E
• coli phages in high throughput laboratories and genomic centers • The φ80 lac ZΔM15 marker that provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and, therefore, can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal • Capable of being transformed efficiently with large plasmids and can serve as a host for the M13mp cloning vectors if a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 is provided to allow plaque formation Genotype F - φ80 lac ZΔM15 Δ(lacZYA- arg F)U169 rec A1 end A1 hsd R17(rk-, mk+) pho A sup E44 λ- thi -1 gyr A96 rel A1 ton A Order Info Shipping Condition: Dry Ice