Invitrogen™ Library Efficiency™ DH5α Competent Cells

Description

• Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications
• Prepared by a proprietary modification of the Hanahan method, these cells are able to achieve transformation efficiencies of >1 x 10 8 cfu/μg control DNA per 100 μL reaction
• Library Efficiency DH5α Competent Cells are recommended for routine cloning of genes into large plasmids and are suitable for difficult clone constructions, such as blunt-end ligations or cloning using limited amount of DNA
• The strain has the lac ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal
• It also carries the rec A1 mutation, which helps reduce the rate of recombination while propagating plasmid DNA, and the end A1 mutation that makes DH5α cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification
• DH5α cells are capable of being transformed efficiently with large plasmids and can serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ (Cat
• No
• 18288019), for example, is provided to allow plaque formation
• Library Efficiency DH5α Competent Cells offer: • Transformation efficiencies of >1 x 10 8 cfu/μg • lac ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal • Plasmid DNA maintained with minimum recombination events due to rec A1 mutation • end A1 mutation that enables increased plasmid yield and quantity • hsd R17 genotype that allows efficient transformation of cloned unmethylated DNA from PCR Genotype F - Φ80 lac ZΔM15 Δ( lac ZYA- arg F) U169 rec A1 end A1 hsd R17 (rk-, mk+) pho A sup E44 λ- thi -1 gyr A96 rel A1 Find the strain and format that fits your needs • DH5α strain and its derivatives are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats
• • MAX Efficiency DH5αF´IQ Competent Cells (Cat
• No
• 18288019) are available in chemically competent format
• • DH5α-T1R competent cells are available in MultiShot format for high throughput applications
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