Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells

Description

• Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 10 6 cfu/μg plasmid DNA
• Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any general application where DNA for transformation is not limiting
• Features of Subcloning Efficiency DH5α Competent Cells • Designed for general, low throughput, everyday use • Standard laboratory strain useful for general cloning applications Ideal for routine subcloning procedures Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors
• 500 μL of chemically competent cells are pre-aliquoted in tubes for ten reactions using 50 μL
• These cells yield >1 x 10 6 transformants/μg control DNA per 50 μL reaction
• Subcloning Efficiency DH5α Competent Cells are not suitable for the generation of cDNA libraries
• Flexible cloning capabilities Benefits of Subcloning Efficiency DH5α Competent Cells include: • lac ZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal • rec A1 that ensures increased insert stability and prevents unwanted recombination • end A1 that improves the yield and quality of plasmid DNA prepared from minipreps • Supports replication of M13mp vectors but not plaque formation Genotype F - Φ80 lac ZΔM15 Δ( lac ZYA- arg F) U169 rec A1 end A1 hsd R17(rk-, mk+) pho A sup E44 thi -1 gyr A96 rel A1 λ- Find the strain and format that you need • We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs
• • Strains are available for challenging cloning applications such as library preparation or cloning unstable DNA
• • SOC medium (Cat
• No
• 15544034) for E
• coli recovery after transformation is available, as well as other ready-to-use bacterial growth formats
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