Invitrogen™ ElectroMAX™ DH5α-E Competent Cells

Description

• ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation
• It is challenging to achieve high transformation efficiency by electroporation using the standard DH5α strain (chemical transformation is more efficient) thus additional mutations were introduced to create the DH5α-E strain that is able to reach >1 x 10 10 efficiency by electroporation with non-saturating amounts of DNA
• Using this strain, 10 to 50 times greater colony number may be obtained in a single transformation than using the chemically competent DH5α cells
• Therefore DH5α-E competent cells are ideal for challenging applications, such as generation of cDNA libraries or in transformations with limited DNA input
• ElectroMAX DH5α-E Competent Cells share several key genetic properties
• They carry the hsd R mutation to prevent restriction of foreign DNA and the end A1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols
• The lac ZΔM15 marker provides α-complementation of the ß-galactosidase gene, allowing blue-white screening on agar plates containing X-gal or Bluo-gal
• DH5α-E allows efficient transformation of large plasmids and can also serve as a host for M13mp cloning vectors if a lawn of DH5αF′IQ is provided to allow plaque formation
• We do not recommended using this strain for direct cloning of methylated genomic DNA
• ElectroMAX DH5α-E Competent Cell features • Greatly increased plasmid yield and quality due to end A1 mutation • High-efficiency transformation with plasmids 30 kb in size • Blue/white screening of recombinant clones due to lac ZΔM15 • Ensured insert stability due to rec A1 mutation • hsdR for efficient transformation of unmethylated DNA from PCR applications Note: A high-voltage electroporation apparatus is required
• Genotype F – φ80 lac ZΔM15 Δ( lac ZYA- arg F)U169 rec A1 end A1 hsd R17(r K – , m K + ) gal pho A sup E44 λ- thi -1 gyr A96 rel A1 Genetic marker descriptions Find the strain and format that fits your needs • DH strains are available in chemically competent and electrocompetent cell formats
• • Explore Multishot format for high throughput applications
• • MAX Efficiency DH5αF´IQ Competent Cells (Cat
• No
• 18288019) are available in chemically competent format
• Order Info Shipping Condition: Dry Ice