18-258-012
Invitrogen™ MAX Efficiency™ DH5α Competent Cells
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-258-012-Each-of-1 | In Stock | ₹ 22,930.08 |
18-258-012 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 22,930.08
GST (18%): ₹ 4,127.414
Total Price: ₹ 27,057.494
Product Type
Chemically Competent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial
No
Blue/White Screening
Yes (lacZΔM15)
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
MAX Efficiency™
Contains F' Episome
No
Cloning Methylated DNA
No
Content And Storage
• Max Efficiency DH5α Competent Cells (5 x 200 μL)Store Competent Cells at –80°C.• pUC19 DNA (100 μL at 0.01 μg/mL)Store pUC19 DNA at –20°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
No
Format
Tube
Quantity
5 x 200 μL
Related Products
Description
- MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications
- Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors
- In addition to supporting blue/white screening, rec A1 and end A1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps
- Benefits of MAX Efficiency DH5α Competent Cells: • Versatile strain for everyday cloning applications • High quality plasmid preparations • Useful for generating plasmid libraries DH5α c ompetent c ells for high transformation efficiency The Φ80 lac ZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal
- DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ (Cat
- No
- 18288019) (or similar) is provided to allow plaque formation
- Features of MAX Efficiency DH5α Competent Cells include: • Transformation efficiency of >1 x 10 9 transformants/μg plasmid DNA • High plasmid yield from the DH5α ( end A1) E
- coli strain • Blue/white screening capable ( lac ZΔM15) • Greater insert stability ( rec A1) Genotype: F- Φ80 lac ZΔM15 Δ( lac ZYA- arg F) U169 rec A1 end A1 hsd R17 (rk-, mk+) pho A sup E44 λ- thi -1 gyr A96 rel A1 Find the strain and format that fits you needs • DH5α strain and its derivatives available in with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats
- • ElectroMAX™ DH5α-E Competent Cells (Cat
- No
- 11319019) are engineered for electroporation to achieve high transformation efficiency of >1 x 10 10
- • DH5α T1R competent cells are available in MultiShot format for high throughput applications
- Order Info Shipping Condition: Dry Ice
Compare Similar Items
Show Difference
Product Type: Chemically Competent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: MAX Efficiency™
Contains F' Episome: No
Cloning Methylated DNA: No
Content And Storage: • Max Efficiency DH5α Competent Cells (5 x 200 μL)Store Competent Cells at –80°C.• pUC19 DNA (100 μL at 0.01 μg/mL)Store pUC19 DNA at –20°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA: Not suitable for cloning unstable DNA
High-throughput Compatibility: Low
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 5 x 200 μL
Product Type:
Chemically Competent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial:
No
Blue/White Screening:
Yes (lacZΔM15)
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
MAX Efficiency™
Contains F' Episome:
No
Cloning Methylated DNA:
No
Content And Storage:
• Max Efficiency DH5α Competent Cells (5 x 200 μL)Store Competent Cells at –80°C.• pUC19 DNA (100 μL at 0.01 μg/mL)Store pUC19 DNA at –20°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA:
Not suitable for cloning unstable DNA
High-throughput Compatibility:
Low
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
5 x 200 μL
Product Type: Chemically Competent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: Library Efficiency™
Contains F' Episome: No
Cloning Methylated DNA: No
Content And Storage: • Library Efficiency DH5α Competent Cells (5 x 200 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA: Not suitable for cloning unstable DNA
High-throughput Compatibility: Low
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 5 x 200 μL
Product Type:
Chemically Competent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Antibiotic Resistance Bacterial:
No
Blue/White Screening:
Yes (lacZΔM15)
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
Library Efficiency™
Contains F' Episome:
No
Cloning Methylated DNA:
No
Content And Storage:
• Library Efficiency DH5α Competent Cells (5 x 200 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA:
Not suitable for cloning unstable DNA
High-throughput Compatibility:
Low
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
5 x 200 μL
Product Type: Chemically Competent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: Subcloning Efficiency (106to 107cfu/μg)
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: Subcloning Efficiency™
Contains F' Episome: No
Cloning Methylated DNA: No
Content And Storage: • Subcloning Efficiency DH5α Competent Cells (4 x 500 μL)Store Competent Cells at –80°C.• pUC19 DNA (20 μL at 100 pg/μL)Store pUC19 DNA at –20°C.
Cloning Unstable DNA: Not suitable for cloning unstable DNA
High-throughput Compatibility: Low
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 4 x 500 μL
Product Type:
Chemically Competent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
Subcloning Efficiency (106to 107cfu/μg)
Antibiotic Resistance Bacterial:
No
Blue/White Screening:
Yes (lacZΔM15)
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
Subcloning Efficiency™
Contains F' Episome:
No
Cloning Methylated DNA:
No
Content And Storage:
• Subcloning Efficiency DH5α Competent Cells (4 x 500 μL)Store Competent Cells at –80°C.• pUC19 DNA (20 μL at 100 pg/μL)Store pUC19 DNA at –20°C.
Cloning Unstable DNA:
Not suitable for cloning unstable DNA
High-throughput Compatibility:
Low
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
4 x 500 μL
Product Type: Lens Cover
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Antibiotic Resistance Bacterial: __
Blue/White Screening: __
Preparing Unmethylated DNA: __
Shipping Condition: __
Species: __
Product Line: __
Contains F' Episome: __
Cloning Methylated DNA: __
Content And Storage: __
Cloning Unstable DNA: __
High-throughput Compatibility: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: __
Quantity: 25/Pk.
Product Type:
Lens Cover
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Antibiotic Resistance Bacterial:
__
Blue/White Screening:
__
Preparing Unmethylated DNA:
__
Shipping Condition:
__
Species:
__
Product Line:
__
Contains F' Episome:
__
Cloning Methylated DNA:
__
Content And Storage:
__
Cloning Unstable DNA:
__
High-throughput Compatibility:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
__
Quantity:
25/Pk.