18-290-015
Invitrogen™ ElectroMAX™ DH10B Cells
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-290-015-Each-of-1 | In Stock | ₹ 31,314.96 |
18-290-015 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,314.96
GST (18%): ₹ 5,636.693
Total Price: ₹ 36,951.653
Product Type
Electrocompetent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Blue/White Screening
Yes (lacZΔM15)
Plasmid
May be used for plasmids >20 kb
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
No
Format
Tube
Quantity
5 x 100 μL
Contains F' Episome
No
Cloning Methylated DNA
Yes (mcrA)
Content And Storage
• ElectroMAX DH10B Cells (5 x 100 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
ElectroMAX™
Related Products
Description
- ElectroMAX DH10B Cells are electrocompetent E
- coli cells offering the highest transformation efficiencies of >1 x 10 10 cfu/μg plasmid DNA
- The high transformation efficiencies make these cells ideal for cloning both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes
- ElectroMAX DH10B Cells are suitable for construction of gene banks, generation of cDNA libraries using plasmid-derrived vectors, and for situations when DNA is limited
- They are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction
- ElectroMAX DH10B Cells provide: • High-efficiency transformation to maximize clones • Enhanced genetic markers for cDNA or gDNA cloning capabilities ElectroMAX DH10B Cell features • Φ80 lac ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal • mcr A genotypic marker and mcr BC, mrr deletion that allow cloning DNA that contains methylcytosine and methyladenine • end A1 mutation that enables increased plasmid yield and quantity Genotype F - mcr A Δ( mrr - hsd RMS- mcr BC) Φ80 lac ZΔM15 Δ lac X74 rec A1 end A1 ara D139Δ( ara , leu )7697 gal U gal K λ - rps L nup G Get more detailed information about specific Genotypes and Genetic Markers of our E
- coli Competent Cells
- Find the strain and format that you need DH10B cells are available in both electrocompetent and chemically competent formats
- ElectroMax DH10B T1R Cells (Cat
- No
- 12033015) and MegaX DH10B T1 R Electrocomp Cells (Cat
- No
- C640003) offer the benefit of T1 and T5 phage resistance
- Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required
- Order Info Shipping Condition: Dry Ice
Compare Similar Items
Show Difference
Product Type: Electrocompetent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Plasmid: May be used for plasmids >20 kb
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 5 x 100 μL
Contains F' Episome: No
Cloning Methylated DNA: Yes (mcrA)
Content And Storage: • ElectroMAX DH10B Cells (5 x 100 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA: Not suitable for cloning unstable DNA
High-throughput Compatibility: Low
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: ElectroMAX™
Product Type:
Electrocompetent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial:
Yes (Streptomycin)
Blue/White Screening:
Yes (lacZΔM15)
Plasmid:
May be used for plasmids >20 kb
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
5 x 100 μL
Contains F' Episome:
No
Cloning Methylated DNA:
Yes (mcrA)
Content And Storage:
• ElectroMAX DH10B Cells (5 x 100 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA:
Not suitable for cloning unstable DNA
High-throughput Compatibility:
Low
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
ElectroMAX™
Product Type: Transfection Reagent
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Antibiotic Resistance Bacterial: __
Blue/White Screening: __
Plasmid: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Quantity: 1 mL
Contains F' Episome: __
Cloning Methylated DNA: __
Content And Storage: Contains one vial (1 ml) of Lipofectin™ Transfection Reagent. Store at 4°C. Do not freeze.
Cloning Unstable DNA: __
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: __
Shipping Condition: Wet Ice
Species: __
Product Line: Lipofectin™
Product Type:
Transfection Reagent
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Antibiotic Resistance Bacterial:
__
Blue/White Screening:
__
Plasmid:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Quantity:
1 mL
Contains F' Episome:
__
Cloning Methylated DNA:
__
Content And Storage:
Contains one vial (1 ml) of Lipofectin™ Transfection Reagent. Store at 4°C. Do not freeze.
Cloning Unstable DNA:
__
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA:
__
Shipping Condition:
Wet Ice
Species:
__
Product Line:
Lipofectin™
Product Type: Transfection Reagent
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Antibiotic Resistance Bacterial: __
Blue/White Screening: __
Plasmid: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Quantity: 4 mL
Contains F' Episome: __
Cloning Methylated DNA: __
Content And Storage: Each unit contains one or multiple vials (1 ml) of reagent. Store at +4°C. Lipofectin™ Transfection Reagent is guaranteed stable for 6 months when stored properly.
Cloning Unstable DNA: __
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: __
Shipping Condition: Wet Ice
Species: __
Product Line: Lipofectin™
Product Type:
Transfection Reagent
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Antibiotic Resistance Bacterial:
__
Blue/White Screening:
__
Plasmid:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Quantity:
4 mL
Contains F' Episome:
__
Cloning Methylated DNA:
__
Content And Storage:
Each unit contains one or multiple vials (1 ml) of reagent. Store at +4°C. Lipofectin™ Transfection Reagent is guaranteed stable for 6 months when stored properly.
Cloning Unstable DNA:
__
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA:
__
Shipping Condition:
Wet Ice
Species:
__
Product Line:
Lipofectin™
Product Type: College Rule Spiral Notebook
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Antibiotic Resistance Bacterial: __
Blue/White Screening: __
Plasmid: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: __
Quantity: __
Contains F' Episome: __
Cloning Methylated DNA: __
Content And Storage: __
Cloning Unstable DNA: __
High-throughput Compatibility: __
Preparing Unmethylated DNA: __
Shipping Condition: __
Species: __
Product Line: __
Product Type:
College Rule Spiral Notebook
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Antibiotic Resistance Bacterial:
__
Blue/White Screening:
__
Plasmid:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
__
Quantity:
__
Contains F' Episome:
__
Cloning Methylated DNA:
__
Content And Storage:
__
Cloning Unstable DNA:
__
High-throughput Compatibility:
__
Preparing Unmethylated DNA:
__
Shipping Condition:
__
Species:
__
Product Line:
__