Invitrogen™ MegaX DH10B T1RElectrocomp™ Cells

Description

• MegaX DH10B T1 R Electrocomp Cells are the highest-efficiency electrocompetent cells available
• Improved production processes provide a transformation efficiency reaching >3 x 10 10 cfu/μg of pUC DNA, making MegaX DH10B T1 R cells ideal for highly demanding cloning or cDNA and gDNA library construction applications
• The DH10B T1 R strain has all the benefits of DH10B genotype, with the addition of the ton A (also known as fhu A) genotype that confers resistance to T1 and T5 phage infection
• The inclusion of the ton A genotype offers added security to protect valuable clones and libraries
• MegaX DH10B T1 R cells carry mutations in the methylation-dependent E
• coli K12 restriction system ( mcr A, mcr BC, and mrr ) allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes
• This strain also has the lac ZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal
• Finally, the end A1 mutation makes DH10B T1 R an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification
• MegaX DH10B T1 R Electrocomp Cells offer: • Highest transformation efficiency of >3 x 10 10 cfu/μg • ton A ( fhuA ) genotype to confer resistance to T1 and T5 phage • lac ZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal • mcr A genotypic marker and the mcr BC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine • end A1 mutation enables increased plasmid yield and quantity • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA Note: A high-voltage electroporation apparatus is required
• Genotype F – mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 end A1 ara D139 Δ( ara - leu )7697 gal U gal K λ rps L(StrR) nup G ton A Find the strain and format that fits your needs • We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs
• • Electroporation cuvettes are available in 0.1 cm (Cat
• No.P41050), 0.2 cm (Cat
• No
• P45050), and 0.4 cm (Cat
• No
• P46050) gaps
• • Strains are available in several MultiShot formats for high throughput applications
• Order Info Shipping Condition: Dry Ice