Invitrogen™ TOP10F' Electrocomp™ Kit

Description

• TOP10F´ Electrocomp Kit E
• coli cells are identical to TOP10 cells, with the addition of an F´ episome
• The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication
• In addition, the F´ episome carries the lac Iq repressor for inducible expression from trc , tac , and lac promoters using IPTG
• TOP10F´ competent cells are transformed via electroporation, thus achieving higher transformation efficiencies than chemically competent cells and may be used in more challenging applications, including cloning a low amount of DNA or cDNA and gDNA library construction
• These cells allow stable replication of high copy number plasmids and are provided at a transformation efficiency of >1 x 10 10 cfu/ μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation
• As a derivative of TOP10, TOP10F´ cells carry mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ) allowing one to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes
• Similar to other DH strains, the TOP10 strain also has rec A1 and end A1 mutations to increase insert stability and improve the quality of plasmid DNA prepared from minipreps
• TOP10F´ Electrocomp Kit E
• coli cells offer: • Transformation efficiencies of >1 x 10 10 cfu/μg • Stable F´ episome that allows isolation of ssDNA and carries lac Iq repressor for inducible expression from trc , tac , and lac promoters • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal • end A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA Note: TOP10F´ cells require IPTG induction for blue-white color screening
• A high-voltage electroporation apparatus is also required
• Genotype F´ [ lac Iq, Tn 10(TetR)] mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara-leu )7697 gal U gal K rps L(StrR) end A1 nup G Find the strains and formats that fit your needs • We offer other DH strains in chemically competent and electrocompetent cell
• Electroporation cuvettes with 0.1 cm (Cat
• No
• P41050), 0.2 cm (Cat
• No
• P45050), and 0.4 cm (Cat
• No
• P46050) gaps are available
• • The TOP10 strain is available in several MultiShot formats for high throughput applications
• • We offer other strains for ssDNA production, such as DH12S (Cat
• No
• 18312017).