C66411
Invitrogen™ TOP10 Electrocomp™ Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | C66411-Each-of-1 | In Stock | ₹ 46,031.28 |
C66411 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 46,031.28
GST (18%): ₹ 8,285.63
Total Price: ₹ 54,316.91
Product Type
Electrocompetent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Blue/White Screening
Yes (lacZΔM15)
Plasmid
May be used for plasmids >20 kb
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
No
Format
Tube
Quantity
10 x 100 μL
Contains F' Episome
No
Cloning Methylated DNA
Yes (mcrA)
Content And Storage
• 10 x 100 μL TOP10 ElectrocompE. coli• 50 μL pUC19 vector (10 pg/μL)Store at –80°C.• 15 mL S.O.C. MediumStore at room temperature.
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
Electrocomp™
Related Products
Description
- TOP10 Electrocomp E
- coli cells are provided at a transformation efficiency of 1 x 10 10 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation
- This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids
- The TOP10 genotype is similar to the DH10B strain and features: • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones • end A1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I • rec A1 for reduced occurrence of non-specific recombination in cloned DNA Notes Please see the product manual for reaction scale recommendations
- A high-voltage electroporation apparatus is required
- Genotype F - mcr A Δ( mrr - hsd RMS- mcr BC ) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara - leu )7697 gal U gal K rps L end A1 nup G Find the strain and format that fits your needs • TOP10 is available in chemically competent and electrocompetent cell formats
- • Choose MultiShot competent cells for high-throughput cloning applications.
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Product Type: Electrocompetent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Plasmid: May be used for plasmids >20 kb
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 10 x 100 μL
Contains F' Episome: No
Cloning Methylated DNA: Yes (mcrA)
Content And Storage: • 10 x 100 μL TOP10 ElectrocompE. coli• 50 μL pUC19 vector (10 pg/μL)Store at –80°C.• 15 mL S.O.C. MediumStore at room temperature.
Cloning Unstable DNA: Not suitable for cloning unstable DNA
High-throughput Compatibility: Low
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: Electrocomp™
Product Type:
Electrocompetent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial:
Yes (Streptomycin)
Blue/White Screening:
Yes (lacZΔM15)
Plasmid:
May be used for plasmids >20 kb
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
10 x 100 μL
Contains F' Episome:
No
Cloning Methylated DNA:
Yes (mcrA)
Content And Storage:
• 10 x 100 μL TOP10 ElectrocompE. coli• 50 μL pUC19 vector (10 pg/μL)Store at –80°C.• 15 mL S.O.C. MediumStore at room temperature.
Cloning Unstable DNA:
Not suitable for cloning unstable DNA
High-throughput Compatibility:
Low
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
Electrocomp™
Product Type: __
Improves Plasmid Quality: __
Transformation Efficiency Level: __
Antibiotic Resistance Bacterial: __
Blue/White Screening: __
Plasmid: __
Reduces Recombination: __
T1 Phage - Resistant (tonA): __
Format: __
Quantity: __
Contains F' Episome: __
Cloning Methylated DNA: __
Content And Storage: __
Cloning Unstable DNA: __
High-throughput Compatibility: __
Preparing Unmethylated DNA: __
Shipping Condition: __
Species: __
Product Line: __
Product Type:
__
Improves Plasmid Quality:
__
Transformation Efficiency Level:
__
Antibiotic Resistance Bacterial:
__
Blue/White Screening:
__
Plasmid:
__
Reduces Recombination:
__
T1 Phage - Resistant (tonA):
__
Format:
__
Quantity:
__
Contains F' Episome:
__
Cloning Methylated DNA:
__
Content And Storage:
__
Cloning Unstable DNA:
__
High-throughput Compatibility:
__
Preparing Unmethylated DNA:
__
Shipping Condition:
__
Species:
__
Product Line:
__
Product Type: Electrocompetent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacΔM15)
Plasmid: May be used for plasmids >20 kb
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): No
Format: Tube
Quantity: 5 x 100 μL
Contains F' Episome: No
Cloning Methylated DNA: Yes (mcrA)
Content And Storage: • 5 x 100μL TOP10 ElectrocompE. coli• 50 μL pUC19 vector (10 pg/μL)Store at –80°C.• S.O.C. Medium (15 mL)Store at room temperature.
Cloning Unstable DNA: Not suitable for cloning unstable DNA
High-throughput Compatibility: Low
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: Electrocomp™
Product Type:
Electrocompetent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1 x 1010cfu/μg)
Antibiotic Resistance Bacterial:
Yes (Streptomycin)
Blue/White Screening:
Yes (lacΔM15)
Plasmid:
May be used for plasmids >20 kb
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
No
Format:
Tube
Quantity:
5 x 100 μL
Contains F' Episome:
No
Cloning Methylated DNA:
Yes (mcrA)
Content And Storage:
• 5 x 100μL TOP10 ElectrocompE. coli• 50 μL pUC19 vector (10 pg/μL)Store at –80°C.• S.O.C. Medium (15 mL)Store at room temperature.
Cloning Unstable DNA:
Not suitable for cloning unstable DNA
High-throughput Compatibility:
Low
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
Electrocomp™
Product Type: Electrocompetent Cells
Improves Plasmid Quality: Yes (endA1)
Transformation Efficiency Level: High Efficiency (>1010cfu/μg)
Antibiotic Resistance Bacterial: __
Blue/White Screening: Yes (lacZΔM15)
Plasmid: May be used for plasmids >20 kb
Reduces Recombination: Yes (recA1)
T1 Phage - Resistant (tonA): __
Format: Tube
Quantity: 10 x 100 μL
Contains F' Episome: __
Cloning Methylated DNA: Yes (mcrA)
Content And Storage: • TOP10F' ElectrocompE. coli(10 x 100 μL)Store at -80°C.• pUC19 vector (50 μL at 10 pg/μL)Store pUC19 DNA at -20°C.• S.O.C. Medium (15 mL)Store at room temperature.
Cloning Unstable DNA: Not suitable for cloning unstable DNA
High-throughput Compatibility: __
Preparing Unmethylated DNA: No
Shipping Condition: Dry Ice
Species: E. coli (K12)
Product Line: Electrocomp™
Product Type:
Electrocompetent Cells
Improves Plasmid Quality:
Yes (endA1)
Transformation Efficiency Level:
High Efficiency (>1010cfu/μg)
Antibiotic Resistance Bacterial:
__
Blue/White Screening:
Yes (lacZΔM15)
Plasmid:
May be used for plasmids >20 kb
Reduces Recombination:
Yes (recA1)
T1 Phage - Resistant (tonA):
__
Format:
Tube
Quantity:
10 x 100 μL
Contains F' Episome:
__
Cloning Methylated DNA:
Yes (mcrA)
Content And Storage:
• TOP10F' ElectrocompE. coli(10 x 100 μL)Store at -80°C.• pUC19 vector (50 μL at 10 pg/μL)Store pUC19 DNA at -20°C.• S.O.C. Medium (15 mL)Store at room temperature.
Cloning Unstable DNA:
Not suitable for cloning unstable DNA
High-throughput Compatibility:
__
Preparing Unmethylated DNA:
No
Shipping Condition:
Dry Ice
Species:
E. coli (K12)
Product Line:
Electrocomp™