Invitrogen™ One Shot™ TOP10 Electrocomp™E. coli

Description

• One Shot TOP10 Electrocomp E
• coli cells are ideal for high-efficiency cloning and plasmid propagation
• Provided at a transformation efficiency of >1 x 10 10 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids
• TOP10 Electrocomp E
• coli cells are transformed via electroporation and achieve higher transformation efficiencies than chemically competent cells
• They can therefore be used in more challenging applications, including cloning low amounts of DNA or cDNA and gDNA library construction
• TOP10 E
• coli cells are genetically similar to the DH10B strain but have been reported to be more resilient to stress conditions like osmotic shock and acidic pH stress
• These cells carry mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ) allowing them to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes
• Similar to other DH strains, TOP10 has the lac ZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal
• One Shot TOP10 Electrocomp E
• coli cells offer: • Transformation efficiencies of >1 x 10 10 cfu/μg • hsd R for efficient transformation of unmethylated DNA from PCR amplifications • mcr A for efficient transformation of methylated DNA from genomic preparations • lac ZΔM15 for blue/white color screening of recombinant clones • end A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I • rec A1 for reduced occurrence of nonspecific recombination in cloned DNA Easy-to-use One Shot format TOP10 Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination
• Genotype F – mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 ara D139 Δ( ara-leu )7697 gal U gal K λ– rps L(Str R ) end A1 nup G Find the strain and format that fit your needs • We offer other DH strains in chemically competent and electrocompetent cell formats
• • Electroporation cuvettes with 0.1 cm (Cat
• No
• P41050), 0.2 cm (Cat
• No
• P45050), and 0.4 cm (Cat
• No
• P46050) gaps are available
• • The TOP10 strain is available in several MultiShot formats for high throughput applications
• Note: TOP10 cells do not require IPTG to induce expression from the lac promoter
• A high-voltage electroporation apparatus is required
• Order Info Shipping Condition: Dry Ice