Invitrogen™ One Shot™ccdB Survival™ 2 T1RCompetent Cells

Description

• Description One Shot ccd B Survival 2 T1 R Competent Cells are suitable for the propagation of plasmids containing the ccd B gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors
• ccd B Survival 2 T1R cells that are made chemically competent can achieve high transformation efficiency of >1 x 10 9 transformants/μg plasmid DNA
• The ccd B Survival 2 T1 R E
• coli strain is derived from the TOP10 strain and offers the same benefits
• Mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ) allow cloning of both prokaryotic and eukaryotic genomic DNA that contains methylcytosine and methyladenine
• This strain also has the lac ZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal
• In addition, rec A1 and end A1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps.The ton A (also known as fhuA ) mutation in ccd B Survival 2 T1 R cells confers resistance to bacteriophages T1 and T5, safeguarding samples against contamination
• ccd B Survival 2 T1 R Competent Cells offer: • >1 x 10 9 transformants/μg efficiency • ton A ( fhuA ) genotype to confer resistance to T1 and T5 phage • Support of high-yield preparation of supercoiled plasmid DNA (the end A1 phenotype) • Reduced nonspecific recombination of cloned DNA (the rec A1 phenotype) Convenient and efficient format One Shot ccd B Survival 2 T1 R E
• coli are supplied in the convenient, single-reaction One Shot format
• Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells
• Genotype: F - mcr A Δ( mrr - hsd RMS- mcr BC) φ80 lac ZΔM15 Δ lac X74 rec A1 ara Δ139 Δ( ara - leu )7697 gal U gal K rps L(Str R ) end A1 nup G fhu A:: IS2 Find the strain and format that fits your needs • We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs
• • MultiShot formats for high throughput applications are available
• Order Info Shipping Condition: Dry Ice