Invitrogen™ One Shot™ TOP10/P3 Chemically CompetentE. coli

Description

• Description One Shot TOP10/P3 Chemically Competent E
• coli cells are designed for vector transformation encoding the synthetic sup F gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other sup F-containing vector preparation
• One Shot TOP10/P3 strain features • P3 plasmid for selection of sup F-containing plasmids • Mutated end A1 gene for increased quality of plasmid DNA preparations • recA1 for reduced occurrence of non-specific recombination in cloned DNA • lacZΔM15 for blue/white color screening of recombinant clones P3 Plasmid The P3 plasmid is a low-copy-number, 60 kb plasmid that carries kanamycin, tetracycline and ampicillin resistance markers
• The kanamycin gene is fully active and is used to select for cells carrying P3
• Tetracycline and ampicillin genes carry amber mutations which render the genes inactive during normal growth and replication of the bacteria
• Upon transformation of a vector carrying the suppressor F gene, the amber mutations in the tetracycline and ampicillin genes on the P3 plasmid are suppressed and the E
• coli are resistant to these antibiotics
• Genotype F - mcrA Δ( mrr-hsdRMS-mcrBC ) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ( ara -leu)7697 galU galK rpsL endA1 nupG P3: KanR AmpR (am) TetR (am) Find the strain and format that you need • TOP10 is offered in chemically competent and electrocompetent cell formats
• • Choose MultiShot competent cells for high-throughput cloning applications.