Invitrogen™ One Shot™ PIR1 Chemically CompetentE. coli

Description

• One Shot PIR1 Chemically Competent E
• coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system)
• The pir gene encodes the replication protein π, which is required to replicate and maintain plasmids containing the R6Kγ origin
• The PIR1 strain contains a mutant allele of the pir gene that maintains the donor vector construct at ∼250 copies per cell
• PIR1 cells are competent and can achieve high transformation efficiency of >1 x 10 9 cfu/μg supercoiled plasmid
• R6Kγ allows tight control and selection and has been used in various applications including genome engineering, mutagenesis studies, bacterial artificial chromosome (BAC) construction, high-capacity extrachromosomal vector systems, and suicide plasmid construction
• One Shot PIR1 Chemically Competent E
• coli cells offer: • pir gene to maintain vectors that contain the R6Kγ ori at ∼250 copies per cell • Transformation efficiencies of >1 x 10 9 cfu/μg • end A1 mutation enables increased plasmid DNA yield and quantity • rec A1 mutation reduce occurrence of unwanted recombination in cloned DNA • hsdR for efficient transformation of unmethylated DNA from PCR applications Convenient and efficient format PIR1 Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• Each tube contains enough cells for one transformation, reducing freeze-thaw cycles and money wasted on unused cells
• Genotype F – ∆lac 169 rpo S(Am) rob A1 cre C510 hsd R514 end A rec A1 uidA(∆Mlu I )::pir-116 Genetic marker descriptions Find the strain and format that fit your needs • Multiple DH strains are available in chemically competent and electrocompetent cell formats
• • We offer PIR2 strain (Cat
• No
• C111110) that carries wild-type pir gene and maintain vectors at ∼15 copies per cell
• • MultiShot formats are available for high throughput applications.