MilliporeSigma™ Novagen™ HMS174 (DE3) pLysS Competent Cells

Description

• HMS174 strains provide the recA mutation in a K-12 background
• Like BLR, these strains may stabilize certain target genes whose products may cause the loss of the DE3 prophage
• DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
• Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
• pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.