MilliporeSigma™ Novagen™ Origami™ B (DE3) Competent Cells

Description

• Origami™ B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG
• Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background
• The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline
• These strains also include the lon and ompT deficiences of BL21, which increase protein stability
• DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
• Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.