MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLysS Singles™ Competent Cells

Description

• Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E
• coli
• These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid
• The tRNA genes are driven by their native promoters
• DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
• Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
• pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.