4530964
Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Reagent, No-Weigh™ Format
Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.
Manufacturer: Fischer Scientific
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Description
- Thermo Scientific™ Pierce Sulfo-SBED Biotin Label Transfer Reagent is a multifunctional reagent for labeling a purified protein and then covalently transferring the attached biotin tag onto specific interactors of that protein
- Ready-to-use kit eliminates the need to prepare the necessary buffers or purchase the critical reagents necessary to perform a label transfer experiment
- Key reagents, Sulfo-SBED and DTT, are supplied in unique, no-weigh, single-use packaging
- Highlights: Sulfo-NHS ester reacts with primary amines at pH 7-9 Aryl azide conjugates randomly when activated with UV light Cleavable disulfide bond allows biotin label transfer Discover protein interactions without radiolabeling Includes: No-Weigh Sulfo-SBED Label Transfer Reagent, 8 × 1mg; BupH Phosphate Buffered Saline, 1 Pk
- (makes 500mL); Label Transfer Buffer (20X), 200mL (makes 4L); Streptavidin-HRP Conjugate, 0.1mg; No-Weigh Dithiothreitol (DTT), 8 × 7.7mg; Slide-A-Lyzer MINI Dialysis Units plus Float, 10K MWCO, 10-100μL, 10 units/Pk.