Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL)

Description

• Remove fusion tags from proteins with the HRV 3C cleavage sequence with Thermo Scientific™ Pierce Human Rhinovirus (HRV) 3C Protease, a recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage
• Additional Information and Online Ordering Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln▾Gly-Pro and cleaves after the glutamine residue
• The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively
• HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design
• Highlights: Effective – HRV 3C is designed to remove purification tags from your protein of interest High activity at 4 ° C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h Flexible – dual-tagged enzyme is compatible with various columns and buffers Includes: Kit contains protease, cleavage buffer and control protein
• Recommended for: Removal of purification tags from fusion proteins