Thermo Scientific™ EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods
Highlights: Pyridyldithiol reacts with -SH groups to form a stable disulfide bond Absorbance of pyridine-2-thione leaving group at 343nm can be used to determine reaction progress Disulfide bond is cleavable using 50mM DTT (Part No
20291) or 100mM β-Mercaptoethanol (Part No
35600) Extended spacer arm prevents steric hindrance Must be dissolved in DMSO or DMF before use Biotinylation occurs between pH 6-9