Molecular Probes™ Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester)

Description

• Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester) is an amine-reactive molecule that enables crosslinking of acrylamide to proteins and other biomolecules containing amines
• The succinimidyl ester of acryloyl-X covalently reacts with amines of proteins, amine-modified nucleic acids, and other biomolecules to yield acrylamides that can be copolymerized into polyacrylamide matrices or on surfaces, such as in microarrays and in biosensors
• For instance, acryloyl-X, SE is used to prepare streptavidin acrylamide (Cat
• No
• S21379), which can copolymerize with acrylamide on a polymeric surface to create a uniform monolayer of immobilized streptavidin for binding to biotinylated ligands such as hybridization probes, enzymes, antibodies, and drugs
• Acryloyl-X, SE is a critical crosslinking reagent used in the super-resolution imaging techniques expansion microscopy (ExM) and protein-retention expansion microscopy (proExM) to add acrylamides to the amines of proteins of a preserved cell or tissue sample
• This crosslinking with acrylamide allows the proteins to be anchored to a polymer hydrogel and polymerized
• The polymer hydrogel is then swelled, allowing the cell or tissue sample to be physically expanded to improve spatial resolution in imaging
• Expansion microscopy is a versatile super-resolution imaging technique that can be performed on many specimens, including 3D imaging of thick samples
• This imaging technique also uses conventional microscopes, enabling easier and faster visualization of nanoscale biological structures without advanced super-resolution microscopes and equipment
• Properties of acryloyl-X, SE: • Synonyms —Acryloyl-X, SE; 6-((acryloyl)amino)hexanoic Acid, Succinimidyl Ester; 2,5-Dioxopyrrolidin-1-yl 6-Acrylamidohexanoate; N-Succinimidyl 6-Acrylamidohexanoate; N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-2-propenamide • Molecular weight (MW) —282.30 • Molecular formula —C 13 H 18 N 2 O 5 • CAS number —63392-86-9 We recommend dissolving in high-quality anhydrous DMSO to attain a stock concentration of up to 10 mM.