Molecular Probes™ Alexa Fluor™ 488 Microscale Protein Labeling Kit

Description

• Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100 μg)
• The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labeling and separation reactions
• Important Features of Microscale Protein Labeling Kits: • Labeled proteins typically ready to use typically in 2 hr (∼30 min hands-on time) • Optimized for 20–100 μg of protein with molecular weights between 12 and 150 kDa • Purified using convenient spin filters with yields between 60 and 90% • Stabilizing proteins must be removed from the sample before labeling Stable Reaction Chemistry and Superior Alexa Fluor™ Dyes In the Microscale Protein Labeling Kits, the reactive dye contains a tetrafluorophenyl (TFP) ester moiety that is more stable in solution than the commonly used succinimidyl (NHS) ester
• TFP esters react efficiently with primary amines of proteins to form stable dye–protein conjugates
• Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10
• And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching
• For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum —Section 1.3
• Learn More About Protein and Antibody Labeling We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup
• See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices
• To learn more about our various kits, read Kits for Labeling Proteins and Nucleic Acids —Section 1.2 in the Molecular Probes™ Handbook
• We’ll Make a Custom Antibody Conjugate for You If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you
• Our custom conjugation service is efficient and confidential, and we stand by the quality of our work
• We are ISO 13485:2000 certified
• For Research Use Only
• Not intended for animal or human therapeutic or diagnostic use.