Molecular Probes™ Alexa Fluor™ 488 Cadaverine

Description

• Alexa Fluor™ 488 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety
• Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line
• Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 488 dye is water soluble and pH-insensitive from pH 4 to pH 10
• In addition to reactive dye formulations, we offer Alexa Fluor™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection
• Detailed information about this AlexaFluor™ cadaverine: • Fluorophore label: Alexa Fluor™ 488 dye • Reactive group: cadaverine • Reactivity: carboxylic acids, aldehydes, and ketones (and glutamine residues through an enzyme-catalyzed transamidation reaction) • Ex/Em of the conjugate: 493/516 nm • Extinction coefficient: 73,000 M -1 cm -1 • Spectrally similar dyes: FITC, GFP • Molecular weight: 640.61 g/mol Cell Tracking and Tracing Applications Alexa Fluor™ cadaverines make excellent fluorescent polar tracers because they are bright, small, and water soluble
• Since they contain an aldehyde-fixable functional group, they can be fixed in cells by treatment with formaldehyde or glutaraldehyde
• They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent (Cat
• No
• I14402)
• Protein Labeling Applications Alexa Fluor™ cadaverines can be used as reactive molecules for adding a fluorescent label to carboxylic acids using a coupling agent such as a carbodiimide; they do not spontaneously react with carboxylic acids in solution
• They do, however, react spontaneously with the common amine-reactive functional groups, including succinimidyl esters and isothiocyanates
• The amine-containing Alexa Fluor™ cadaverines can also be used to label glutamine residues in some proteins and peptides via an enzyme-catalyzed transamidation reaction
• Learn More About Protein and Antibody Labeling We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup
• See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices
• To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids–Section 1.2 in The Molecular Probes™ Handbook
• We’ll Make a Custom Conjugate for You If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you
• Our ustom conjugation service is efficient and confidential, and we stand by the quality of our work
• We are ISO 13485:2000 certified
• Dissolve in water to prepare a stock concentrations of 1 to 10 mg/mL
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