Invitrogen™ Click-IT™ AHA (L-Azidohomoalanine)

Description

• Click-iT AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein
• AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis
• Detection utilizes the chemoselective ligation or 'click' reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT Protein Analysis Detection Kits containing either TAMRA, Dapoxyl™, or biotin alkyne
• Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS/MS and MALDI MS analysis or Multiplexed Proteomics™ reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein
• Less hazardous Cell Analysis, Click Chemistries, Labeling Chemistry, Metabolic Labeling and Nascent Protein Synthesis with Click Chemistry, Protein and Antibody Labeling, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Room temperature