Molecular Probes™ FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins

Description

• Description The FluoReporter™ Biotin Quantitation Assay Kit provides a sensitive fluorometric assay for determining the number of biotin labels on nucleic acid samples (i.e., cDNA)
• The assay uses Biotective™ Green reagent, which contains a fluorescent dye that is quenched by ligands occupying the biotin binding sites
• In the presence of biotin, the quencher dye ligands are displaced and fluorescence occurs
• The fluorescence is proportional to the amount of added biotin
• The results can be read with any fluorescence-based microplate reader capable of detecting FITC dye
• Important Features of FluoReporter™ Biotin Quantitation Assay Kits: Can detect from 4 to 80 picomoles of biotin in a sample (50-fold higher sensitivity than HABA biotin-binding assay) Can be applied to as little as 13 ng of biotin-labeled nucleic acid (more is needed for lower degrees of labeling) Excitation/emission maxima of the reagent is 495/519 nm Supplied with a biotinylated positive control for standard curve For Research Use Only
• Not intended for human or animal therapeutic or diagnostic use.